Rhomboid proteases in ER-associated degradation (12)

TP12研究菱形蛋白酶RHBDL4如何在内质网（ER）和分泌途径中解决和避免蛋白质毒性应激。Lemberg团队将基于细胞的研究与蛋白质组学、膜生化和低温电镜相结合，旨在破译rhbdl4催化裂解的分子机制。在第二个工作流程中，TP12将采用组织培养模型和来自RHBDL4敲除小鼠的原代细胞相结合的方法，研究RHBDL4在应激反应中整合和调节分泌动态的功能。

TP12 investigates how the rhomboid protease RHBDL4 resolves and avoids proteotoxic stress in the Endoplasmic Reticulum (ER) and secretory pathway. By combining cell-based studies with proteomics and membrane biochemistry with cryo-electron microscopy, the Lemberg group aims to decipher the molecular mechanism of RHBDL4-catalyzed cleavage. In a second work stream, TP12 will use a combination of tissue culture models and primary cells from RHBDL4 knockout mice to study the function of RHBDL4 in integrating and regulating secretion dynamics in stress response.