Regulation of epidermal stratification and homeostasis through contractility and density sensing (A11)

哺乳动物表皮复杂而刻板的组织结构是如何通过生长、分化和细胞定位的协调来形成、维持和再生的，这是生物学中一个基本的开放问题。我们观察到，肌动蛋白细胞骨架产生和感知的机械力是整合组织拓扑和单细胞命运的表皮动态平衡的关键调节因素。我们将破译细胞黏附的动态变化，通过对张力的影响来驱动层化，并确定肌动球蛋白机制调节表皮谱系识别基因表达的分子机制。

How the complex but stereotyped tissue structure of the mammalian epidermis is formed, maintained and regenerated through coordination of growth, differentiation and cell positioning is a fundamental open question in biology. We observe that mechanical forces generated and sensed by the actomyosin cytoskeleton are critical regulators of epidermal homeostasis by integrating tissue topology with single cell fate. We will decipher how dynamic changes in cell adhesion, through effects on tension, drive stratification and identify the molecular mechanisms by which the actomyosin machinery regulates expression of epidermal lineage identity genes.