Impact of the Neuropeptide S system and NPS-receptor polymorphisms on fear and anxiety (A08)

项目A08的目的是人类相关的NPSR1多态性（N107I）的功能表征。利用CRISPR/ cas9介导的基因编辑技术，一个类似的突变（I107N）被引入到一个新的小鼠模型中。这种突变对神经元特性、恐惧相关大脑区域（如杏仁核、层内丘脑）的神经回路、恐惧消退和泛化的功能后果将在体外/离体使用电生理和光遗传学技术以及在体内使用已建立的行为和压力范式进行分析。

Aim of the project A08 is the functional characterization of the human-relevant NPSR1 polymorphism (N107I). An analogous mutation (I107N) has been introduced into a new mouse model using CRISPR/Cas9-mediated gene editing. The functional consequence of this mutation on neuronal properties, on neuronal circuits in fear relevant brain regions (e.g. amygdala, intralaminar thalamus), fear extinction and generalization will be analyzed in vitro/ex vivo using electrophysiological and optogenetic techniques and in vivo using established behavioral- and stress-paradigms.