GRK 1986: Complex Scenarios of Light-Control

RTG(Complex Light Control-“CLIC”)的目标是教育博士生在光控制化学和生物过程中的光保护策略的综合实现、理论描述、光化学表征和生物分子应用。当空间或时间方面在实验中很重要时，光是理想的外部触发信号。利用耐光性基团可以获得高度精确的控制水平，这可以防止正确的折叠或反应，直到用激光脉冲“取消”的时刻。在第一个资助期的开创性工作的基础上，我们将继续开发越来越复杂的单光子，特别是双光子可光“笼子”群。后者允许高精度的三维光控，分辨率低至几个毫微升。在原理证明之后，我们将继续研究新的Viper去化，在这种情况下，笼组的微小变化可能会导致意外的寻址选择性。这些策略将被示范地应用于DNA和RNA折叠的光调节、膜蛋白反应的控制、受体聚集、伴侣功能、免疫抑制物、时间分辨质谱学和蛋白质合成抑制剂。本RTG的教学课程对于在这一方向进行高影响力的研究至关重要，因为它将为快速而成功的博士阶段提供必要的跨学科教育支持和通用技能。RTG的重要元素将是博士生群体与PI的高度一致性、密切的监督、结构化的科学互动、微调的通用技能课程选择、客座科学家计划以及我们的暑期学校。因此，我们将确保为博士生提供最佳的研究教育，他们将接受合成、理论描述、光谱方法和高级光学显微镜方面的培训，以便在学术界和工业界进行不同的职业选择。

The goal of RTG (Complex Light Control – “CLiC”) is to educate PhD students in the synthetic realization, theoretical description, photochemical characterization and biomolecular application of photolabile protecting strategies for the control of chemical and biological processes with light. Light is an ideal external trigger signal whenever spatial or temporal aspects are important in an experiment. Highly precise levels of control can be obtained with a photolabile group, which prevents correct folding or reactivity until the moment of “uncaging” with a laser pulse. Building on the groundbreaking work of the first funding period, we will continue developing more and more sophisticated one- and especially two-photon photolabile “caging” groups. The latter allow a highly precise three-dimensional photocontrol with resolutions down to few femtoliters. After the proof of principle, we will continue to investigate the new VIPER uncaging in which minute changes in caging groups can lead to unforeseen addressing selectivity. These strategies will be exemplarily applied to the photoregulation of DNA- and RNA-folding, the control of membrane protein reactions, receptor-clustering, chaperone function, immune suppressors, time-resolved mass spectrometry and protein synthesis inhibitors.The teaching curriculum of this RTG is vital to conduct high impact research in this direction, as it will provide the necessary educational support across disciplines along with the generic skills for a fast and successful PhD phase. Important elements in the RTG will be the high coherence of the group of PhD students together with the PIs, a close supervision, structured scientific interaction, a fine-tuned selection of courses in generic skills, the guest scientist program as well as our Summer Schools. Thus, we will ensure an optimal research education for PhD students who will obtain training in synthesis, theoretical description, spectroscopic methods and advanced light microscopy for diverse career options in academia and industry.