Magnetic hybridmaterials in regenerative medicine: production, simulation, application and toxicologic investigations

在这个SPP中，实验肿瘤学和纳米医学部(SEON)主要负责与临床相关的项目。我们的工作集中在研究组织状环境中或人体真实基质中的磁性粒子。我们打算与SPP内外的不同合作伙伴共同致力于以下项目：使用磁性杂化材料生成血管移植。我们正在开发新型可内皮化血管支架。这些支架将使用磁场内皮化，将磁性颗粒-细胞杂交物引导到内表面。这些人工支架应该提供一个紧密而稳定的内表面，由内皮细胞和平滑肌细胞两个不同的细胞层组成。最初，支架将由聚四氟乙烯、聚乳酸或明胶组成。内皮化将使用磁性细胞播种技术进行。在实验中收集的经验将有助于实现这些新材料长期稳定的细胞群的快速有效的方法。磁性纳米颗粒与基于纤维蛋白的网络的相互作用-血栓溶解的研究。我们正在研究一种利用磁场治疗急性血栓形成的新概念。这将深入了解微粒之间的相互作用和血栓的弹性网络，并可能为未来治疗血栓提供基础。其目的是研究是否有可能利用静磁场或振荡磁场将磁性溶栓结合杂化材料引导到血栓中，从而从内部溶解网络。这将极大地降低大剂量溶栓治疗的风险，并极大地改善治疗的预后。纳米颗粒和磁性杂化材料与内皮屏障的相互作用。对于药物的运输和纳米颗粒的生物分布来说，重要的是它们是否能够穿透或损伤内皮屏障。因此，必须研究这些材料的毒性和对内皮屏障的潜在损害。因此，我们将使用紧密生长的上皮细胞以及平滑肌细胞和上皮细胞的多层共培养进行实验。此外，我们还将使用脑屏障和胎盘模型进行实验。我们将研究粒子如何穿透生物屏障，以及稳定日冕在迁移过程中是否发生变化。

In this SPP the Section for Experimental Oncology and Nanomedicine (SEON) is mainly occupied with clinical relevant projects. Our work concentrates on the investigation of magnetic particles in a tissue-like environment or in a real matrix of the human body. Together with different partners within and outside the SPP we intend to work on the following projects:Generation of vessel transplants using magnetic hybrid materials.We are working on the generation of novel endothelializable vessel scaffolds. Those scaffolds will be endothelialized using magnetic fields directing magnetic particle-cell hybrids to the inner surface. Those artificial scaffolds should provide a tight and stable inner surface consisting of two different cell layers of endothelial and smooth muscle cells. In the beginning the scaffold will be composed of PTFE, PLGA or gelatin. The endothelialization will be performed using the magnetic cell seeding technique. The experience collected during the experiments will help to achieve a fast and efficient way for a long-time stable cell population of these new materials.Interactions of magnetic nanoparticles with fibrin-based networks - Investigation of thrombolysis.We are working on a new concept for the treatment of acute thrombosis using magnetic fields. This will provide a deep understanding of the interactions between particles and the elastic network of a thrombus and might provide the basis for future treatments of blood clots. The goal is to investigate whether it is possible to direct magnetic thrombolytica-binding hybrid materials into a thrombus using static or oscillating magnetic fields, thus dissolving the network from within. This would strongly reduce the risks of a systemic treatment with high doses of thrombolytica and extremely improve the prognosis of the treatment.Interaction of nanoparticles and magnetic hybrid materials with endothelial barriers.Important for the transport of drugs and the biodistribution of nanoparticles is, whether they are able to penetrate or injure endothelial barriers. Thus the toxicity of the materials and the potential to harm the endothelial barriers must be investigated. Therefore we will perform experiments using tightly grown epithelial cells as well as multilayered co-cultures of smooth muscle cells and epithelial cells. Additionally we will perform experiments using brain-barrier and placenta models. We will investigate how particles penetrate biological barriers and whether the stabilizing corona changes during migration.