Arbuscular mycorrhiza along forest soil sequences differing in P-availability

贸易平衡模型假设，丛枝菌根（AM）植物变得越来越依赖于它们的真菌共生体作为P的可用性下降。因此，AM协会的重要性可能会增加低磷可用性的生态系统。通过AM群落测量和土壤磷池的详细评估相结合，我们希望获得一个全新的见解池可以访问的基石共生体，如AM真菌。除此之外，我们期待新的结果的多样性和丰富性，这组生物在这个“非经典”的生态系统，这些共生体。我们假设，AM真菌，作为主要代理商的磷收购在许多植物中，将增加丰富度和多样性（在土壤和根）沿着磷耗尽序列在林下，因此将越来越多地有助于磷循环。为了这个目的，我们的目标是评估除了AMF丰度（菌丝长度和根定植）AMF的多样性，通过焦磷酸测序。这可能会刺激检查其他这样的“非传统”系统，也许他们也有丰富的未知AM真菌的多样性。一个额外的温室实验将使我们能够量化的磷吸收量的植物通过AMF菌丝，据我们所知，这是从来没有在这种类型的生态系统。

The trade balance model postulates that arbuscular mycorrhizal (AM) plants become increasingly dependent on their fungal symbionts as P availability declines. Consequently, the importance of AM associations may be expected to increase in ecosystems of low P availability. Through the combination of AM community measurements and detailed assessment of soil P pools, we expect to gain a radically new insight into which pools can be accessed by keystone symbionts like AM fungi. Beyond this we expect novel results on the diversity and abundance of this group of organisms in this ‘non-classical’ ecosystem for these symbionts. We hypothesize that AM fungi, as main agents of P acquisition in many plants, will increase in abundance and diversity (in the soil and roots) along the P depletion sequences in the understory, and will hence increasingly contribute to P recycling. For this aim we aim to assess in addition to AMF abundance (hyphal length and root colonization) AMF diversity via pyrosequencing. This may stimulate examination of other such ‘non-traditional’ systems; perhaps they, too, harbor a wealth of unknown AM fungal diversity. An additional greenhouse experiment will allow us to quantify the amount of P uptake by the plant via AMF hyphae, which to our knowledge was never done in this type of ecosystems.