Histone H4K20 demethylation in cell cycle-controlled differentiation (A12)

我们目前从模式生物非洲爪蟾中得到的结果表明，在增殖细胞中，组蛋白H4K20me1和-me2水平受s期稀释的调节，而在有丝分裂后分化细胞中，组蛋白去甲基化酶（如PHF8、RSBN1和HR23B）会调节组蛋白水平。通过结合外胚层类器官培养中RNA-Seq和ChIP-Seq方法的功能增益和损失分析，我们计划剖析这些酶对平衡胚胎H4K20甲基化的重要性，以及这对表皮细胞在发育过程中成功分化的重要性。

Our current results from the model organism Xenopus indicate that Histone H4K20me1 and -me2 levels are regulated by S-phase dilution in proliferating cells, while becoming adjusted by specific histone demethylases such as PHF8, RSBN1 and HR23B in postmitotic, differentiating cells. By combining gain and loss of function analysis with RNA-Seq and ChIP-Seq approaches in ectodermal organoid cultures, we plan to dissect, how important these enzymes are for balancing the embryonic H4K20 methylation landscape, and how this is crucial for the successful differentiation of epidermal cells during development.