MRI: Acquisition of Flexible High-speed Super-Resolution System for Plant Research

唐纳德·丹福斯植物科学中心(DDPSC)被授予对植物进行高速和超分辨率显微镜研究，以了解它们与微生物和环境的相互作用。DDPSC的使命是通过植物科学改善人类状况：喂饱饥饿的人，改善人类健康；保护和更新环境；加强圣路易斯地区作为世界植物科学中心的地位。DDPSC在先进显微镜方面的投资极大地有利于其植物研究项目，并为更大的科学界提供了以植物为重点的新应用。这种超分辨率显微镜将被用来培训各级科学家，直至主要研究人员。圣路易斯大学和圣路易斯华盛顿大学的三门研究生/本科生课程将在教学大纲中包括超分辨率显微镜。未来的科学家将通过积极参与DDPSC NSF-本科生研究体验(REU)计划、教授一年两次的高级荧光成像短期课程、为成像项目招募高中生以及参加名为学生和教师的地区性实习计划(STAR)来培训。此外，非常成功的DDPSC教育和外联计划将为教师举办研讨会，并提供互动指导，将我们的科学家与附近的学校联系起来，包括圣查尔斯社区学院和哈里斯托大学，这是一所历史上的黑人学院和大学。近年来，得益于基因编码荧光传感器和其他设计者探测器的快速发展，关于细胞如何工作的新发现即将出现显著激增。此外，新的成像技术和协议已经出现，导致了如何探索细胞和组织的革命。DDPSC对高速超分辨率显微镜的收购将对23个得到资助的研究项目产生立竿见影的影响，这些项目包括NSF(12个)、DOE(4个)、DARPA(1个)、USDA(2个)、PRIVAL(3个)、NIH(1个)，代表丹福斯中心和当地的13个PI，包括华盛顿圣路易斯大学(WUSTL)、圣路易斯大学(SLU)和堪萨斯州立大学(KSU)。该超分辨率显微镜将在植物发育、微生物、植物-微生物相互作用、植物和藻类的热/应激反应、植物和藻类细胞生长和分裂的基本原理、细胞骨架动力学、植物细胞壁、核域结构和动力学、RNA定位以及植物或藻类组织中的蛋白质定位等方面取得进展。这项研究的结果将在同行评议的出版物和科学会议上传播。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

An award is made to the Donald Danforth Plant Science Center (DDPSC) to do high-speed and super-resolution microscopy of plants to understand their interactions with microbes and their environment. The DDPSC mission is to improve the human condition through plant science: by feeding the hungry and improving human health; preserving and renewing the environment; and enhancing the St. Louis region as a world center for plant science. The investment in advanced microscopy at the DDPSC greatly benefit its plant research projects and provides novel plant focused applications for the greater scientific community. The super-resolution microscope will be leveraged for training scientists at all ranks up to principal investigators. Three graduate/undergraduate courses at Saint Louis University and Washington University in Saint Louis will include super-resolution microscopy in their syllabus. Tomorrow’s scientists will be trained through active participation in the DDPSC NSF-Research Experiences for Undergraduates (REU) Program, teaching a twice-yearly advanced fluorescence imaging short course, recruitment of high school students for imaging projects and participation in a regional internship program for high school students called Students and Teachers As Research Scientists (STARS). Additionally, the highly successful DDPSC Education and Outreach program will host workshops for teachers and provide interactive mentoring that connect our scientists with nearby schools including St. Charles Community College and Harris Stowe University, a Historically Black College and University.In recent years, a remarkable surge in new discoveries on how cells work have been forthcoming, benefiting from the rapid development of genetically encoded fluorescent sensors and other designer probes. Additionally, new imaging technologies and protocols have arisen, leading to a revolution in how a cells and tissues can be explored. DDPSC acquisition of a high-speed super-resolution microscope will have an immediate impact on twenty-three funded research projects NSF (12), DOE (4), DARPA (1) USDA (2), private (3), NIH (1) representing 13 PIs at the Danforth Center and local region including Washington University of Saint Louis (WUSTL), Saint Louis University (SLU) and Kansas State University (KSU). The super-resolution microscope will enable advancements in diverse projects which investigate plant development, microbes, plant-microbe interactions, heat/stress responses in plants and algae, fundamentals of plant and algal cell growth and division, cytoskeletal dynamics, the plant cell wall, nuclear domain structure and dynamics, RNA localization, and protein localization in plant or algal tissues. Results from the research will be disseminated in peer-reviewed publications and at scientific meetingsThis award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.