MRI: Acquisition of an Octet RED96 BioLayer Interferometry Instrument for Multidisciplinary Molecular Interaction Research and Training

授予南达科他州州立大学（SDSU）功能基因组学核心设施（FGCF）以获得和实施Octet RED 96生物层干涉仪（BII）。该BII将使来自整个南达科他州（SD）的研究人员和学生能够研究生物分子相互作用速率（即动力学），包括DNA-蛋白质，蛋白质-蛋白质和蛋白质-小分子相互作用，涉及现有和未来的研究项目。这些分子相互作用的动力学是所有生物过程的基础，因此量化这些相互作用对于SD生物科学研究的发展至关重要。了解相关技术将促进大多数生物学科现有和未来科学家的专业发展。FGCF的使命是通过为研究人员和教育工作者提供尖端分子技术的机会和培训，促进病毒、细菌、植物和动物系统的跨学科基因功能研究和培训。FGCF目前没有BII系统提供的功能，但该设施的用户需要这些功能。作为FGCF的一部分，Octet BII将立即加强至少三所SD大学的集体研究工作，涉及15名研究人员，34名研究生，28名本科生和6名博士后和访问科学家。这些研究工作主要集中在改善人类和动物健康，并尽量减少作物生产对环境的影响。来自可持续发展机构的教师也致力于将BII技术纳入几门研究生和本科生课程。FGCF还将负责促进这些技术在该地区所有学院、大学和新兴公司的各种可持续发展研究计划中的利用。该仪器还将允许BII技术被纳入正在进行的推广计划，如高中教师研讨会，通过SDSU Wokini Initiative为美国原住民学生提供的培训计划以及通过REU计划提供的夏季本科生研究经验。农业植物中的共生微生物关系，哺乳动物免疫系统中的优化功能成分，以及各种病毒-受体特异性相互作用。这一工具将增强新兴研究实体的实力（例如，生物化学时空网络资源（BioSNTR）和2D材料生物膜工程科学和技术（2DBEST）研究中心，旨在利用先进的成像和分子生物学技术研究涉及细胞信号传导的复杂分子回路）通过添加工作流程来定量测量和动力学表征各种生命系统中的生物分子相互作用。BioSNTR是之前NSF EPSCOR RII计划的重点，该计划题为“2020年愿景：为南达科他州建立研究、教育和创新伙伴关系”，但是它的活动正在通过新的项目进行，这些项目将由这种仪器来促进。这个奖项反映了美国国家科学基金会的法定使命，并且通过利用基金会的智力价值和更广泛的影响审查进行评估，被认为是值得支持的的搜索.

An award is made to South Dakota State University (SDSU) Functional Genomics Core Facility (FGCF) to acquire and implement an Octet RED96 BioLayer Interferometry Instrument (BII). This BII will enable researchers and students from throughout the state of South Dakota (SD) to study biomolecular interaction rates (i.e. kinetics), including DNA-protein, protein-protein, and protein-small molecule interactions across a variety of biological system involving existing and future research projects. The kinetics of these molecular interactions are the basis for all biological processes, and so quantifying these interactions is critical for the growth of bioscience research in SD. Understanding the associated technologies will promote professional development of existing and future scientists within most biological disciplines. The mission of the FGCF is to facilitate interdisciplinary gene function research and training in viral, bacterial, plant and animal systems by providing researchers and educators with access to and train for cutting-edge molecular technologies. The capabilities provided by a BII system is not currently available in the FGCF but are needed by the users of this facility. As a component of the FGCF, the Octet BII will immediately enhance the collective research efforts from at least three SD universities involving 15 investigators, 34 graduate students, 28 undergraduate students, and 6 postdoctoral associates and visiting scientists. These research efforts are primarily focused on improving human and animal health and minimizing the environmental impact of crop production. Teachers from the SD institutions have also committed to incorporating BII technologies into several graduate and undergraduate courses. The FGCF will be responsible for also promoting the utilization of these technologies into various SD research programs within all colleges, universities and emerging companies within the region. This instrument will also allow BII technologies to be incorporated into ongoing outreach programs, such as high-school teacher workshops, training programs for Native American students through the SDSU Wokini Initiative and summer undergraduate research experiences through REU programs.There are a wide variety of projects ready to use this instrument with studies ranging from nitrogen fixation in cyanobacteria, to the symbiotic microbe relationships in agricultural plants, to the optimizing functional components within mammalian immune systems, and various virus-receptor specific interactions. This instrument will enhance strengths among emerging research entities (e.g. the Biochemical Spatiotemporal NeTwork Resource (BioSNTR) and 2D material Biofilm Engineering Science and Technology (2DBEST) research centers established to investigate the sophisticated molecular circuits involved in cell signaling using advanced imaging and molecular biology technologies) by adding a workflow to quantitative measure and kinetically characterize biomolecular interactions in various living systems. The BioSNTR was the focus of a previous a NSF EPSCOR RII program entitled “The 2020 Vision: Building Research, Education, and Innovation Partnerships for South Dakota", but it's activities are ongoing through new projects that will be facilitated by this instrumentation.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.