MRI: Acquisition of a FACSMelody Flow Cytometer and Cell Sorter

爱达荷州立大学被授予获得一台荧光激活细胞分类器(FACS)的流式细胞仪。该仪器将极大地增加爱达荷州立大学和邻近的本科院校北洋理工大学多个系和学院的研究和教育机会。该仪器将被安置在一个多用户核心设施中，供生物科学、药学和人类学的研究人员和教师使用。将在各种本科生和研究生课程以及中等教育教师培训课程中开发和使用动手实验设计模块。通过将这种设备融入课堂，各级教育的学生将在真实的研究活动中获得实践经验。社区合作伙伴也可以使用这种设备，并将其纳入大学范围内的外展和STEM培训活动，以促进多样化劳动力的招聘、留住和专业发展。从本科生到教授，各种研究人员都将使用这种仪器。仪器设置的简便性和跨多学科应用的多样性将增强当前的研究能力，并为新的和创新的项目提供机会。研究人员将使用这台机器回答以下问题：1)免疫细胞对环境污染物和生理刺激的反应；2)生物和细胞发育过程中细胞蛋白质的表达；3)植物基因组/倍体的量化；4)动物组织中激素受体的表达；5)原核生物中的基因表达；6)细胞分裂和死亡；以及7)考古样本(如牙齿、陶器)中的微粒鉴定。这些领域中的许多都依赖于这台机器的分选功能来分离活细胞或小颗粒，以便进一步实验。使用该仪器产生的数据将通过演示文稿和同行评审论文向公众传播，数据将影响广泛的领域，同时为学生提供环境科学、发育生物学、进化生物学、真核和原核细胞生物学以及人类学方面的尖端培训。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

An award is made to Idaho State University to acquire a Fluorescence-Activated Cell Sorter (FACS) flow cytometer. This instrument will greatly enhance the research and educational opportunities across multiple departments and colleges at Idaho State University and at near-by undergraduate institution BYU-I. The instrument will be housed in a multi-user core facility and accessed by researchers and teachers in the Biological Sciences, Pharmaceutical Sciences, and Anthropology. Hands-on experimental design modules will be developed and utilized in various undergraduate and graduate courses, and within secondary education teacher-training courses. By incorporating this equipment into classes, students at all levels of education will gain hands-on experience in authentic research activities. This equipment will also be accessible to community partners and incorporated into university-wide outreach and STEM training activities to promote recruitment, retention, and professional development of a diverse workforce.This instrument will be utilized by a variety of researchers, from undergraduate students to professors. The ease of instrument set-up and diversity of applications across multiple disciplines will enhance current research capabilities and open opportunities for new and innovative projects. Researchers will use this machine to answer questions pertaining to 1) immune cell responses to environmental contaminants and physiologic stimuli, 2) cellular protein expression during organismal and cellular development, 3) plant genome/ploidy quantification, 4) hormone receptor expression in animal tissues, 5) gene expression in prokaryotes, 6) cell division and death, and 7) microparticle identification in archeologic samples (i.e., teeth, pottery). Many of these areas rely on the sorting functions of this machine to isolate live cells or small particles for further experimentation. Data generated using this instrument will be disseminated to the public via presentations and peer-reviewed papers, and data will impact a wide array of fields while providing cutting-edge training for students in environmental sciences, developmental biology, evolutionary biology, eukaryotic and prokaryotic cell biology, and anthropology.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.