I-Corps: Cyanobacterial enzymes and strains for removal of organophosphonates

该I-Corps项目更广泛的影响/商业潜力是开发低成本的可喷洒，快速作用的酶/微生物，将有机膦酸酯降解为环境友好的中间体或最终产品，减少这些农用化学品对环境的污染。膦酸盐的使用增加（作为清洁剂、冷却剂和杀虫剂等）由于其缓慢的降解性和由此产生的在环境中的积累，所提出的技术寻求选择性地降解包括草甘膦除草剂的有机膦酸酯。 拟议的项目将支持发展战略，安全和有效地使用农用化学品和家用化学品的各种基本applications.This I-Corps项目是基于生产的三个磷酸酶过表达他们在快速增长的蓝藻菌株。这些酶可用于去除土壤和水系统中的膦酸盐污染物。来自阿肯色州温泉的嗜热蓝细菌物种也被鉴定为高水平表达这些酶的菌株。该项目旨在利用有机膦酸酯降解的新机制，并帮助清洁环境。几种蓝藻可能能够生存的有机膦酸盐作为唯一的磷源，分解有机膦酸盐。这个奖项反映了NSF的法定使命，并已被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估的支持。

The broader impact/commercial potential of this I-Corps project is the development of low-cost sprayable, fast-acting enzymes/microorganisms that degrade organophosphonate into environmentally-friendly intermediates or final products, reducing envirionmental pollution from these agrochemicals. The increased use of phosphonates (as cleaning agents, coolants, and pesticides, etc.) is problemmatic due to their slow degradability and resultant accumulation in the environment. The proposed technology seeks to selectively degrade organophosphonates including glyphosate herbicides. The proposed project will support the development of strategies for safe and effective use of agrochemicals and household chemicals for various essential applications.This I-Corps project is based on the productio of three phosphonatases by overexpressing them heterogously in fast-growing cyanobacterial strains. These enzymes can be used for the removal of phosphonate contaminants in soil and water systems. Thermophilic cyanobacteria species from Hot Springs, Arkansas are also identified as strains expressing these enzymes in high levels. This projects seeks use the novel mechanisms of organophosphonate degradation and to help clean the environment. Several cyanobacterial species may be able to survive on organophosphonate as the only source of phosphorus, breaking down organophosphonates.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.