Functional relevance of the Rho signaling inhibitor Myosin 9B and downstream cytoskeletal proteins for cutaneous dendritic cell populations (B01)

本项目研究细胞骨架成分与皮肤DC功能的相关性。特别是，进化上保守的白细胞特异性运动蛋白Myo9B和下游的细胞骨架效应分子cofilin(控制F-肌动蛋白周转)和Fasin-1(F-肌动蛋白束，在DC中特异表达)的功能作用将通过使用转基因DC来阐明。不同的皮肤病模型(利什曼病、牛皮癣、半抗原和蛋白质特异性接触性皮炎和耐受诱导)的病程将使用DC中缺乏Fasin-1或Myo9B的小鼠进行评估。

This project investigates the relevance of cytoskeletal components for cutaneous DC functions. In particular, the functional roles of the evolutionarily conserved leukocyte-specific motor protein Myo9B and the downstream cytoskeletal effector molecules Cofilin (governs F-actin turnover) and Fascin-1 (bundles F-actin, specifically expressed in DC) will be elucidated by using genetically modified DC. The course of distinct cutaneous disease models (leishmaniasis, psoriasis, hapten- and protein-specific contact dermatitis and tolerance induction) will be assessed using mice deficient for Fascin-1 or for Myo9B in DC.