Relationship of pollination ecology to population genetic structure and speciation rates in Justicia (Acanthaceae)

不同传粉媒介在花粉携带和覆盖距离方面的不同行为预计会导致种群之间不同程度的基因流动。这将导致具有不同授粉综合征的种群之间种群遗传结构的差异，并最终导致不同的物种形成或灭绝率。然而，这一假设从未得到有效的验证。在拟议的研究中，假定的因果链的所有三个环节——传粉、种群遗传结构、多样化率——将首次被一起研究。选择的研究小组是新世界司法，一个单系组包含蜜蜂和蜂鸟授粉的代表。我们将对三种嗜虫和三种嗜虫进行染色花粉授粉实验，以验证花粉流差异的假设。本文将对三种嗜虫和三种嗜虫进行分子分析，以验证种群遗传结构差异的假设。随后，将对新世界正义属进行基于序列的系统发育分析，以验证具有不同授粉综合征的姐妹枝的多样化率始终不同的假设。

Divergent behaviour of different pollinating agents in terms of pollen carry-over and distances covered is expected to result in different degrees of gene flow between populations. This would then lead to differences in population genetic structure between populations with different pollination syndromes, and ultimately in divergent speciation or extinction rates. This hypothesis, however, has never been effectively tested.In the proposed study, all three links of the presumed causal chain ¿ pollination, population genetic structure, diversification rates ¿ will for the first time be examined together. The chosen study group is New World Justicia, a monophyletic group containing bee and hummingbird pollinated representatives. Pollination experiments with dyed pollen will be conducted on three melittophilous and three trochilophilous species to test the hypothesis of differences in pollen flow. A molecular analysis of three melittophilous and three trochilophilous species will be conducted to test the hypothesis of differences in population genetic structure. Subsequently, a sequence-based phylogenetic analysis of New World Justicia will be conducted to test the hypothesis of consistently divergent diversification rates in sister clades with different pollination syndromes.