Mechanisms governing polar localization and assembly of the host cell targeting type VI secretion system (B16*_beant. B13)

细胞被膜的一个重要功能是正确定位和展示细胞表面毒力因子，以便它们与宿主细胞相互作用。本研究的目的是深入了解跨细胞膜VI型分泌系统5（T6 SS-5）的细菌细胞极的放置机制。使用有针对性的和随机诱变和（活）荧光显微镜，除其他外，我们将研究T6 SS-5蛋白到达极点的相互依赖性，以及候选细胞因子（如细胞骨架）和新因子在T6 SS-5正确定位中的作用。

An important function of the cell envelope is to correctly position and display cell surface virulence factors for their interaction with the host cell. The goal of this study is to gain an insight into the mechanisms underlying the placement of the cell envelope spanning type VI secretion system 5 (T6SS-5) to the bacterial cell pole. Using targeted and random mutagenesis and (live) fluorescence microscopy, among others, we will investigate the co-dependence of the T6SS-5 proteins to reach the pole as well as the role of candidate cellular factors -such as the cytoskeleton- and of novel factors in the proper positioning of the T6SS-5.