Proteomic platform

蛋白质组平台将是研究联合体的组成部分，并进行鉴定和验证项目P1、P2和P3的新型膜内蛋白酶底物所需的大量定量质谱学测量。该联盟的所有项目都需要质谱学分析的结果，例如P7中的比较生物信息学分析。该平台拥有两台高分辨率Orbitrap质谱仪和相关设备，包括纳米LC，并拥有所需方法的专业知识，如无标记定量和不同的含同位素标记方法。这包括体外和体内的“细胞培养中氨基酸稳定同位素标记”(SILAC)分析。

The proteomic platform will be an integral part of the research consortium and performs the numerous quantitative mass spectrometry measurements required for identification and validation of novel intramembrane protease substrates for projects P1, P2 and P3. The results of the mass spec analyses will be required for all projects in the consortium, e.g. for the comparative bioinformatic analyses in P7. The platform has two high resolution Orbitrap mass spectrometers plus associated equipment, including nanoLCs, and has expertise with the required methods, such as label free quantification and different isotope label-containing methods. This includes 'stable isotope labelling with amino acids in cell culture' (SILAC) analyses in vitro and in vivo.