Serine ubiquitination and regulation of autophagy upon Legionella (E01 (C01))

我们将研究丝氨酸泛素化对宿主自噬途径的影响以及这可能如何影响军团菌的细菌感染。我们将进行功能研究来阐明军团菌SidE蛋白和RavZ在损害宿主自噬中的作用。我们将使用超分辨率显微镜研究含军团菌液泡周围的纳米级蛋白质组织，重点关注自噬，信号蛋白和泛素化。我们将开发一个功能，时空和分子图像的过程，以保护军团菌自噬介导的清除。

We will examine the impact of serine ubiquitination on the host autophagic pathway and how this might affect bacterial infection by Legionella. We will perform functional studies to elucidate the contribution of Legionella SidE proteins and RavZ in impairing host autophagy. We will use super-resolution microscopy to study the nanoscale protein organization around the Legionella-containing vacuole, focusing on autophagy, signaling proteins, and ubiquitination. We will develop a functional, spatiotemporal and molecular picture of processes that guard Legionella from autophagy-mediated clearance.