Elucidation of the dynamics of the autophagosomal membrane-associated protein GABARAP by NMR spectroscopy (B03)

虽然自噬是细胞蛋白质降解的基本机制之一，但自噬体膜区室的调节仍然不完全清楚。使用国家的最先进的NMR光谱与其他生物物理和生物化学技术相结合，我们将调查的构象动力学和功能机制的GABARAP，一个关键的自噬相关的蛋白质，交替之间的可溶性形式在细胞质和膜锚定的形式，以介导膜拴系和半融合在自噬体生物发生。

Although autophagy is one of the fundamental cellular protein degradation mechanisms, the regulation of autophagosomal membrane compartments is still not fully understood. Using state-of-the-art NMR spectroscopy in concert with other biophysical and biochemical techniques we will investigate the conformational dynamics and functional mechanism of GABARAP, a key autophagy-related protein which alternates between a soluble form in the cytosol and a membrane-anchored form to mediate membrane tethering and hemifusion during autophagosome biogenesis.