Dynamics of proteins of the inner nuclear membrane (P07)

细胞核被内核膜和外核膜（INM， ONM）包围，它们在核孔水平相连，核孔是细胞核和细胞质之间的通道。在这个项目中，我们想要研究inm蛋白，其中一个重点是VAPB，一种以前被描述为在许多接触部位发现的er驻留蛋白的蛋白质。我们研究了新型vapb与INM蛋白和核孔复合物相互作用的功能相关性，我们使用了一种新的基于接近度的筛选方法。此外，我们计划通过生命细胞显微镜分析VAPB（和其他inm蛋白）的动力学，并研究与肌萎缩性侧索硬化症家族型相关的VAPB突变形式。

The nucleus is surrounded by the inner and the outer nuclear membrane (INM, ONM), which are connected at the level of the nuclear pores, the gates between the nucleus and the cytoplasm. In this project, we want to investigate INM-proteins, with one focus on VAPB, a protein that has previously been described as an ER-resident protein found in many contact sites. We investigate the functional relevance of novel VAPB-interactions with proteins at the INM and the nuclear pore complex that we identified using a novel, proximity-based screening approach. Furthermore, we plan to analyse the dynamics of VAPB (and other INM-proteins) by life-cell microscopy and to investigate a mutant form of VAPB that is involved in a familial form of amyotrophic lateral sclerosis.