Elucidation of the mutation rate as driver of insect endosymbiont genome evolution

在这个项目中，我们的目标是研究Blattabacterum基因组的进化。为此，我们计划对许多Blattabacterum基因组进行测序。我们已经测序了448个Blattabacterum基因组。虽然其中一些没有组装成单一的重叠群，但超过250个组装成了重叠群。对于那些没有在单个重叠群中测序的基因，我们计划在2023财年进行额外的测序。我们获得的所有完整基因组都已被注释，我们正在分析它们。我已经为这个项目发表了两篇科学论文。一篇论文发表在《分子生物学与进化》(IF：16.2)，一篇论文发表在《分子系统发生与进化》(IF：4.3)。这些论文的参考文献如下：Kinso Y.*，Lo N.*(与第一作者相同)，Villa-Martin P.，Tokuda G.，Pigolotti S.和Bourguignon T.(2021)在蟑螂内共生菌Blattabacterium中，突变率提高、宽松选择和多米诺骨牌效应与基因丢失有关。《分子生物学与进化》，38,3820-3831。车勇，邓伟，李伟，张军，金田勇，德田，布尔吉尼翁，罗宁，王正(2022)。从线粒体基因组和核基因(18S，28S)推断的替代和扩散事件塑造了全球隐尾藻的分布。分子系统发育与进化166号，107318号。取得的成绩与原计划一致。

In this project, we aim to study the evolution of Blattabacterium genomes. We planned to sequence many Blattabacterium genomes for that purpose. We have sequenced 448 Blattabacterium genomes. While some of them were not assembled in a single contig, over 250 were. For those that were not sequenced in a single contig, we are planning extra sequencing during FY2023. All the full genomes we obtained have been annotated, and we are in the process of analyzing them.I have published two scientific papers for this project. One paper was published in Molecular Biology and Evolution (IF: 16.2), and one paper was published in Molecular Phylogenetics and Evolution (IF: 4.3). The references of these paper are as follow: Kinjo Y.*, Lo N.* (*equal first authors), Villa-Martin P., Tokuda G., Pigolotti S. & Bourguignon T. (2021) Enhanced mutation rate, relaxed selection, and the ‘domino effect’ are associated with gene loss in Blattabacterium, a cockroach endosymbiont. Molecular Biology and Evolution, 38, 3820-3831. Che Y., Deng W., Li W., Zhang J., Kinjo Y., Tokuda G., Bourguignon T., Lo N. & Wang Z. (2022). Vicariance and dispersal events inferred from mitochondrial genomes and nuclear genes (18S, 28S) shaped global Cryptocercus distributions. Molecular Phylogenetic and Evolution 166, 107318. The achievements are as originally planned.