Identification and characterization of photosynthetic and translational modulators involved in chloroplast acclimation to low temperature (A04)

我们以前发现了几个由叶绿体编码的非必需基因，它们是冷适应的候选调节器。现在，我们将阐明这些基因的调控以及它们在光合作用和翻译的冷驯化中的生理作用。此外，我们将通过全细胞核糖体图谱来分析翻译的冷反应，以获得这一过程的定量、密码子解析的图像，并识别其他翻译调节的调节因子和冷适应的整合因子。此外，我们还将揭示冷驯化过程中叶绿体翻译机制的组成动态，以揭示核糖体蛋白和核糖体相关因子的调节功能。

We previously identified several plastid-encoded non-essential genes as candidate modulators of cold acclimation. Now, we elucidate the regulation of these genes and their physiological role in cold acclimation of photosynthesis and translation. Furthermore, we will analyse the cold response of translation by whole-cell ribosome profiling to obtain a quantitative, codon-resolved picture of this process and to identify additional translationally regulated modulators and integrators of cold acclimation. Moreover, we will unravel the compositional dynamics of the plastid translation machinery during cold acclimation to reveal regulatory functions of ribosomal proteins and ribosome-associated factors.