The role of OEP16 and OEP40 in cold acclimation (B05)

我们最近发现了两个叶绿体外膜定位的溶质通道，即OEP16.1和OEP40，它们也参与了拟南芥的冷驯化。我们的目标是了解OEP16.1和OEP40如何在冷适应过程中对代谢网络和信号转导做出贡献。这将通过1.通过详细了解它们的生化特性，例如底物特异性、门控和蛋白质结构的调节，以及2.通过分析在驯化过程中依赖于体内OEP16.1和OEP40的转录、翻译后和代谢变化，以细胞器和组织特异性的定量方式来实现。

We have recently identified two chloroplast outer envelope localized solute channels, namely OEP16.1 and OEP40, which are also involved in cold acclimation in Arabidopsis. Our goal is to understand how OEP16.1 and OEP40 contribute to metabolic networking and signalling during cold acclimation. This will be achieved 1. by obtaining a detailed understanding of their biochemical properties, e.g. substrate specificity, regulation of gating and protein structure, and 2. by analysing the transcriptional, post-translational and metabolic changes, which depend on OEP16.1 and OEP40 in vivo in an organelle and tissue-specific quantitative manner during the acclimation process.