Multienzymatic Cascade in a 2-Phase System - Process integration

生物催化作为一种替代性的化工生产工艺，在化工生产中的地位越来越重要.在这种情况下，多酶级联反应有可能满足对具有高选择性和产物纯度的复杂合成路线的需求，并且不需要在中间进行产物分离。特别是体外系统具有高时空产率的潜力，尽管它们不太复杂，数学描述相对简单，因此易于优化。在2相系统中成功建立多酶级联反应后，计划通过工艺集成进行强化。为此目的，级联的反应步骤与萃取步骤和随后的两相分离在一个装置中组合。因此，第一台三相（l/l/s）反应萃取离心机问世。这一独特的设备同时也是第一台酶反应提取离心机。这一高度创新的项目旨在促进平台技术的发展，将通常单独进行的联合收割机工艺步骤结合起来。因此，可以更好地理解生物催化中复杂的集成过程。该系统将被描述的数学和建模。最后，根据系统的各个步骤，给出了一个完整的过程模型，为过程优化提供了有价值的帮助。

Biocatalysis becomes more and more important as an alternative production process in chemical industry. In this context multi-enzyme cascade reactions have the potential to meet the demand for complex synthesis routes with high selectivity and purity of the products and no need for product isolation in between. Especially in-vitro systems have a potential for high space-time yields, although they are less complex, comparatively simple to describe mathematically and therefor good to optimize. Following the successful establishment of a multi-enzyme cascade reaction in the 2-phase system, intensification by process integration is planned. For this purpose, a reaction step of the cascade is combined with the extraction step and the subsequent separation of the two phases in one apparatus. Therefore, the first 3-phase (l/l/s) reactive extraction centrifuge is introduced. This unique apparatus is at the same time the first enzymatic reactive extraction centrifuge.This highly innovative project aims to contribute the development of a platform technology to combine process steps, which are usually individually performed. Thus, a better understanding of complex integrated processes in biocatalysis can be achieved. The system will be described mathematical and modelled. In the end a whole process model will be given, based on the individual system steps, to give a valuable assistance for process optimization.