Preparation of anti-Arsenic based Antibodies and Development of ELISA for Arsenic Analysis in the Environment

砷通常存在于环境中的各种化学物种中，这些化学物种的毒性和流动性各不相同。人类通过饮用水和食物接触到相当数量的砷，并在某种程度上通过受污染的空气暴露，具体取决于地理区域。由于这些对健康的关注，近年来，随着了解砷在环境中的存在、分布和动员以及开发经济有效的处理技术的需要，砷的研究稳步增长。新的仪器技术正在快速发展，以努力产生更低的检测下限、更短的时间分辨率和更高的选择性的分析方法，目的是增强我们对砷的去向和传输的了解。一般而言，现场可展开的仪器可能是更好的选择，因为它使我们能够进行实时分析。免疫分析技术提供了一种新的方法，与传统的仪器密集型金属分析方法相比具有显著的优势。该方法具有快速、简便、高通量等特点，适用于现场分析。本研究的目的是制备抗砷抗体。这将用于开发酶联免疫吸附分析(ELISA)，以便在不同的样本中，例如在亚洲的水样中，方便和廉价地分析这些物种。

Arsenic is commonly found throughout the environment in a wide variety of chemical species that vary in toxicity and mobility. Humans are exposed to a considerable amount of arsenic through drinking water and food and to some extent, through contaminated air depending on geographical areas. As a result of these health concerns, arsenic research has grown steadily in recent years in accord with the need to understand the occurrence, distribution, and mobilization of arsenic in the environment and to develop cost-effective treatment technologies.New instrumental techniques are being developed at a rapid rate in an effort to produce analytical methods with lower detection limits, shorter temporal resolution, and increased selectivity with the goal of enhancing our understanding of fate and transport of arsenic. In general, a field deployable instrument can be of better choice since it enables us to do real-time analysis. Immunoassay technology offers a new approach and has significant advantages over the traditional instrument-intensive methods of metals analysis. They are remarkably quick, easy, high throughput and feasible for field analysis. The aim of the present research is to prepare antibodies against arsenic species. This will be used in development of Enzyme-linked Immunosorbent Assay (ELISA) to allow easy and cheap analysis of these species in different samples, e.g. in water samples in Asia.