NanoSIMS

哥廷根校区是欧洲成像技术和生物医学科学的领先中心之一，重点是神经科学。我们目前能够通过电子显微镜、X射线显微镜和最现代的光成像技术来跟踪细胞和细胞器。这些工具使我们能够研究细胞结构的组织和运动。然而，他们忽略了时间维度，即细胞寿命期间发生的变化（细胞周转）。这方面可以通过将同位素标记的代谢物掺入活细胞和动物中来解决，这应该以高精度成像。因此，我们在这里申请一种能够以高分辨率成像同位素物质的仪器：在横向平面中为30-50 nm，在轴向方向上低至小于6 nm。该仪器将用于各种项目，主要是生物医学领域，但也用于地质学，化学和物理学。结合哥廷根校区目前的成像专业知识，该设备将为德国研究提供新的资源：以德国生物医学NanoSIMS设施的形式对代谢和周转进行高分辨率成像。

The Göttingen Campus is one of the leading centers in Europe in both imaging technology and biomedical sciences, with an emphasis on neuroscience. We are currently able to follow cells and organelles by electron microscopy, X-ray microscopy, and by the most modern light imaging techniques. These tools enable us to investigate the organization and the movement of cellular structures. However, they ignore the temporal dimension, the changes that take place during the cellular lifetime (cellular turnover). This aspect can be addressed by the incorporation of isotopically-tagged metabolites into living cells and animals, which should be imaged with high precision. We therefore apply here for an instrument capable of imaging isotopic species at high resolution: 30-50 nm in the lateral plane, and down to less than 6 nm in the axial direction. This instrument will be used in a variety of projects, mostly in the biomedical field, but also in geology, chemistry and physics. Combined with the current expertise in imaging at the Göttingen campus, this device will provide a new resource for German research: the high-resolution imaging of metabolism and turnover, in the form of a German Biomedical NanoSIMS Facility.