Apical cell growth and generation of cellular asymmetry in corynebacteria (P05)

谷氨酸棒状杆菌是研究根尖生长的模式生物。顶细胞壁合成机制的时空组织和染色体的极性系固是由支架蛋白DivIVA控制的。棒状菌具有异常复杂的细胞包膜，称为mycolyl -阿拉伯半乳糖-肽聚糖（mAGP）复合物。我们将用DivIVA分析mAGP合成的空间组织。DivIVA还被鉴定为ParB-parS核蛋白复合物的极性系链。新复制的ParB复合物是如何形成的，姐妹起源的分离过程是如何开始的，这些问题仍然没有解决。我们将在新的资助期内处理ParB复杂的形成和动态。此外，我们将揭示一种新的、diviva定位的dna修复机制的功能。我们的工作将提供对细菌根尖生长的细胞生物学的全面认识，并有助于了解根尖生长与染色体组织的密切联系。

Corynebacterium glutamicum is a model organism to study apical growth. The spatiotemporal organization of apical cell wall synthesis machineries and polar tethering of the chromosome are governed by the scaffold protein DivIVA. Corynebacteria have an unusually complex cell envelope termed mycoloyl-arabinogalactan-peptidoglycan (mAGP) complex. We will analyze the spatial organization of the mAGP synthesis by DivIVA. DivIVA was also identified as polar tether for the ParB–parS nucleoprotein complex. It remains unsolved how newly replicated ParB complexes are forming and how the segregation process of sister origins is initiated. We will address ParB complex formation and dynamics in the new funding period. Furthermore, we will unravel the function of a novel, DivIVA-localized DNA-repair mechanism. Our work will provide a comprehensive insight into the cell biology of an apically growing bacterium and contribute to the understanding of the intimate connection of apical growth on chromosome organization.