MALDI-TOF-TOF Massenspektrometer

质谱成像（MSI）使用空间分辨蛋白质组学和代谢组学技术来说明直接在患者组织样品上的数百个生物分子的同时空间分布。近年来，MALDI（基质辅助激光解吸/电离）-成像在组织分析特别是脑组织探测中的重要性显著增加，其在探索伴随神经退行性疾病的脑蛋白质组变化方面的优势已在不同的研究中显示。样品可以直接分析，不需要额外的化合物提取步骤，样品的标记也很简单。质谱仪器的空间分辨率是限制质谱组织成像技术发展的因素之一。 激光束直径可实现高空间分辨率，此外，激光的软电离允许检测完整的蛋白质，并使该仪器有资格用于分子成像和临床研究。

Mass spectrometry imaging (MSI) uses spatially resolved proteomic and metabolomic techniques to illustrate the simultaneous spatial distributions of hundreds of biomolecules directly on patient tissue samples. Last years the importance of the MALDI (Matrix-assisted laser desorption/ionization)-Imaging in tissue analysis especially in brain tissue exploration has increased significantly, and its advantage for the exploration of the brain proteome changes that accompany the neurodegenerative disease have been shown in different studies. The samples can be directly analyzed; no need of additional extraction steps of compounds and the labeling of the sample is dispensable. The spatial resolution of mass spectrometry instruments is one of the limiting factors in advancing the mass spectrometric tissue imaging. The laser beam diameter enables high spatial resolution, moreover, the soft ionization of the laser allows the detection of intact proteins and qualifies this instrument for molecular imaging and for clinical research ambitions.