The effects of luteotrophic and luteolytic factors on feline luteal cells (functional characterization)

大多数猫科动物被认为是濒危物种。为了保护物种，还必须阐明繁殖的生物学基础。因此，莱布尼茨动物园和野生动物研究所（莱布尼茨- izw）多年来一直在研究各种领域的生殖生物学。我们能够证明，在猞猁，黄体（CL）在（假）怀孕后仍然活跃，这是一种生殖特性。在本项目中，我们正在对猫科动物黄体细胞进行促黄体和促黄体因子的检测。在本课题的第一部分中，我们认识到起始材料的局限性和异质性导致实验的可行性有限。因此，在第二部分（延续项目）中，我们希望使用黄体细胞的祖细胞进行长期细胞培养，并希望使用这些特征化的细胞在3D模型中进行黄体化。然后，我们将使用这些模型来测试活性氧（ROS）和抗氧化物质对黄体细胞活力的影响。如果我们通过这些研究更好地了解CL活力的控制，这不仅与猫科动物有关，而且与其他物种CL不足的治疗有关。

The majority of felids are considered as endangered. In order to contribute to the conservation of species, the biological basis of reproduction must also be elucidated. The Leibniz Institute for Zoo and Wildlife Research (Leibniz-IZW) has therefore been studying the reproductive biology of various felids for many years. We were able to show that in lynxes, corpora lutea (CL) are still active after (pseudo-)pregnancy, which is a reproductive peculiarity. In the project, we are testing luteotropic and luteolytic factors on luteal cells of feline species. In the first part of this project on this topic, we recognized that the limitation and heterogeneity of the starting material led to a limited feasibility of experiments. Therefore, in the second part (continuation project) we would like to use the progenitor cells of the luteal cells for the development of long-term cell cultures and would like to use these characterized cells for luteinization in 3D models. We will then use the models to test the influence of reactive oxygen species (ROS) and antioxidant substances on luteal cell viability. Should we gain a better understanding of the control of CL viability through these studies, this will not only have relevance for felids, but could also be relevant for the treatment of CL insufficiencies in other species.