マイクロ流路デバイス中の流れと空間の制御による超高速イムノセンシングの実現

当一个人患有某种疾病时，生物标志物的数量就会升高。这项研究的重点是开发敏感和快速的微流体系统，以检测各种生物标志物。在这里，动力学增强微流控生物测定系统进行了研究，使用新型的3D玻璃设备。分析荧光标记的受体和配体抗体之间的实时结合事件。结合实验测量与标度分析，提出了两个关键的控制无量纲参数，以实现“快速”和“灵敏”的配体检测：本地Peclet数和动力学Damkohler数。最后，我们总结了我们的观察，提供设计规则，以支持快速和灵敏的微流控生物检测系统的发展。

When a person is afflicted with a disease, quantities of biomarkers are elevated. This research is focused on developing sensitive and rapid microfluidic systems to detect a wide range of biomarkers. Here, kinetic enhancements in microfluidic bioassay systems were investigated using novel 3D glass devices. Real-time binding events between fluorescently-labelled receptor and ligand antibodies were analyzed. Combining experimental measurements with scaling analysis, two key control dimensionless parameters were proposed to achieve “rapid” and “sensitive” ligand detection: a local Peclet number and a kinetic Damkohler number. Finally, we summarized our observations by providing design rules to support the development of fast and sensitive microfluidic bioassay systems.