SICOPID_Activation and regulation of plasma membrane receptor signaling complexes controlling plant development and immunity, and their connection to downstream signaling cascades

植物是大自然中另一个成功的多细胞生命实验。为了协调细胞、组织和器官的生长和发育，植物进化出了独特的质膜受体激酶（RKs）。该蛋白质家族的几个成员作为模式识别受体和塑造植物结构的激素受体发挥作用。越来越多的证据表明，不同的植物RKs组织在膜信号复合物。RK具有共同的结构架构并且共享下游信令组件。因此，目前尚不清楚细胞表面特异性内源性或外源性信号的识别如何转化为胞质溶胶中特异性发育程序或免疫应答的激活。我们建议将联合收割机生理学、遗传学和细胞生物学与磷酸蛋白质组学、定量生物化学和结构生物学相结合，以确定植物发育和免疫受体复合物被激活的共同和特定机制。我们将剖析，在分子上的细节，如何激活受体复合物产生特定的信号输出在胞质溶胶和植物RKs的活性是如何调节抑制蛋白。我们设想，我们的工作将提供一个分子框架，以了解特异性是如何在分子水平上编码RK信号，为未来在作物中设计这些途径奠定基础。

Plants are natures other successful experiment with multicellular life. To coordinate growth and development of their cells, tissues and organs plants have evolved unique plasma membrane receptor kinases (RKs). Several members of this protein family function as pattern recognition receptors, and as hormone receptors shaping the architecture of the plant. There is mounting evidence that different plant RKs are organized in membrane signaling complexes. RKs have a common structural architecture and share downstream signaling components. As such, it is presently unclear how the recognition of specific endogenous or foreign signals at the cell surface is translated into the activation of specific developmental programs or immune responses in the cytosol. We propose to combine physiology, genetics and cell biology with phosphoproteomics, quantitative biochemistry and structural biology to identify the shared and specific mechanisms by which plant developmental and immune receptor complexes are activated. We will dissect, in molecular detail, how activated receptor complexes generate specific signaling output in the cytosol and how the activity of plant RKs are regulated by inhibitor proteins. We envision that our work will provide a molecular framework for understanding how specificity is encoded at the molecular level in RK signaling, setting the stage for engineering these pathways in crops in the future.