Structure and dynamics eukaryotic 80S ribosomes during the elongation phase of protein synthesis (A03)

在A03项目中，真核生物的核糖体延伸周期将使用高分辨率的单粒子冷冻电镜进行结构分析。核糖体举例说明了作为大分子机器组织的功能模块。由可调节的能量景观控制的结构动力学促进了核糖体的功能。为了在分子水平上获得真核核糖体延伸周期的结构基础，将对体外组装的核糖体复合物以及体外衍生的多体进行研究。此外，这将揭示大分子机器内部工作的一些一般原理。

In project A03 the ribosomal elongation cycle in eukaryotes will be structurally analyzed using high-resolution single-particle cryo-EM. The ribosome exemplifies functional modules that are organized as macromolecular machines. Structural dynamics governed by a tuneable energy landscape facilitates ribosomal function. To obtain the structural foundation of the eukaryotic ribosomal elongation cycles at the molecular level in vitro assembled ribosomal complexes as well as ex vivo derived polysomes will be investigated. Moreover, this will reveal some general principles about the inner workings of macromolecular machines.