Mitochondrial haplotypes and phenotypes in laying hens

线粒体参与了几个生物体的关键过程，因此，深入研究有效利用磷和肌醇代谢的母鸡mtDNA单倍型和mt基因表达是至关重要的。肌醇通过与自噬和有丝分裂吞噬相关的未知机制来调节线粒体的功能。在拟议的项目中，我们将获得两个不同品系的蛋鸡的完整线粒体基因组单倍型，这些单倍型是在拟议的研究单位内的另一个项目的实验中获得的。这种方法确定了在蛋鸡遗传选择过程中出现的影响关键酶活性的潜在突变。为了测试mt基因在不同单倍型之间的表达差异，将进行组织和发育特异性定量(Q)聚合酶链式反应实验。我们将我们的数据与研究股其他项目中获得的基因组分析的数据结合起来，以找到相互作用的基因座之间的联系，这取决于核背景。此外，线粒体DNA单倍型与有关代谢组学特征的分子信息有关，特别是与酰肉碱作为线粒体功能指标有关，在另一个项目中进行了评估。因此，我们将全面了解线粒体在不同植酸和磷利用以及肌醇代谢的母鸡中的功能重要性。

Mitochondria are involved in several organismic key processes; hence the in-depth characterization of mtDNA haplotypes and mt gene expression in hens with efficient phosphorus (P) utilization and myo-inositol metabolism is of utmost importance. Myo-inositol is known to modulate mitochondrial functionality by so far unknown mechanisms related to autophagy and mitophagy. In the proposed project we will obtain whole mitochondrial genome haplotypes for two distinct strains of laying hens obtained in experiments of another project within the proposed Research Unit. This approach determines potential mutations that arose during the genetic selection process in laying hens affecting key enzyme activities. To test for mt gene expression differences among distinct haplotypes, tissue and developmental-specific quantitative (q) PCR experiments will be performed. We combine our data with those of genome analyses obtained in other projects of the Research Unit to find associations of interacting loci depending on the nuclear background. Further, mtDNA haplotypes are associated with the molecular information regarding metabolomics profiles, especially with acylcarnitines as indicators of mitochondrial function, assessed in another project. Thus, we will gain comprehensive insights into the functional importance of mitochondria in hens varying in phytate and P utilization and myo-inositol metabolism.