Quantitative fluorescence microscopy for the analysis of Wnt pathway interactions and dynamics (A06)

在这个合作项目中，Wnt配体相互作用的量化将扩展到所有10个FZD受体，从而获得关于Wnt天然结合选择性及其决定Wnt信号特异性的重要的、未解决的信息。FFS方法(例如，轴线扫描FCS)将被用于量化参与信号体形成的配体-受体和受体-受体的相互作用。通过超分辨率单分子定位显微成像(SMLM)，可以显示Wnt受体/辅受体簇和信号小体在质膜上形成的化学计量比和动力学。该项目既依赖于新技术，也依赖于财团内部的跨学科合作。

In this collaborative project the quantification of Wnt ligand interactions will be extended to all ten FZD receptors, thereby gaining important, unresolved information on the native binding selectivity of a Wnt and its potential to dictate Wnt signaling specificity. FFS approaches (e.g., axial line-scanning FCS) will be used to quantify ligand-receptor and receptor-receptor interactions involved in signalosome formation. By super-resolution single-molecule localization microscopy imaging (SMLM) the stoichiometry and dynamics of Wnt receptor/co-receptor clusters and signalosome formation at the plasma membrane will be visualized. The project relies on both novel technologies and interdisciplinary collaborations within the consortium.