Role of SUMOylation of Satb2 in the regulation of ES cell differentiation, gene expression and higher-order chromatin structure (P04)

该项目解决了通过SUMO修饰可以改变高阶染色质结构调节剂Satb2功能特异性的机制。我们之前已经证明Satb2在胚胎干细胞（ES）的多能性调控和B细胞免疫球蛋白重链基因位点的高阶染色质结构中发挥重要作用。最近，我们发现在胚胎干细胞分化过程中，Satb2被新的SUMO E3连接酶Znf451介导。在下一个资助期，我们将研究znf451介导的Satb2 summoylation如何改变Satb2调节ES细胞多能性的功能。

This project addresses the mechanisms by which the functional specificity of Satb2, a regulator of higher-order chromatin structure, can be altered by SUMO modification. We have previously shown that Satb2 plays important roles in the regulation of pluripotency of embryonic stem (ES) cells and in the higher-order chromatin structure of the immunoglobulin heavy chain gene locus in B cells. Recently, we found that Satb2 is SUMOylated during differentiation of ES cells by the novel SUMO E3 ligase Znf451. In the next funding period we will examine how Znf451-mediated SUMOylation of Satb2 alters the function of Satb2 in regulating ES cell pluripotency.