Protein modifications and interactions of the FOXO/DAF-16 and mTORC mediated stress response (P05)

我们重点研究了胰岛素/IGF和雷帕霉素靶蛋白（mTORC）信号通路中关键成分的蛋白修饰和相互作用。血清和糖皮质激素诱导的激酶SGK-1通过其动态定位和广泛的相互作用以及对其关键成分的修饰将这两条途径结合在一起。在此，我们在线虫和细胞培养模型中提出了遗传和蛋白质生化方法，以了解应激和营养如何通过FOXO转录因子DAF-16和mTORC2相互作用因子Rictor调节SGK-1的反馈调节。

We focused on the identification of protein modifications and interactions of key components in insulin/IGF and target-of-rapamycin (mTORC) signaling. The serum-and-glucocorticoid-inducible kinase SGK-1 couples both pathways via its dynamic localization and extensive interactions and modifications of its key components. Here, we propose genetic and protein-biochemical methods in C. elegans and cell culture models to understand how stress and nutrition modulate the feedback regulation of SGK-1 via the FOXO transcription factor DAF-16 and the mTORC2 interactor Rictor.