Hybrid Quadrupol Massenspektrometer

基于质谱的定量蛋白质组学是研究组织、细胞和亚细胞蛋白质组以及蛋白质翻译后修饰的有力技术。这项技术彻底改变了许多生物学领域的蛋白质水平和修饰的量化，其中包括时间生物学。生物钟是一种内源性计时系统，以日常方式调节生理功能和行为。生物钟通过有节奏地调节基因表达和蛋白质功能来发挥其控制作用。虽然昼夜节律转录调控被广泛研究，但对蛋白质功能的昼夜节律动力学的理解仍处于起步阶段。我在过去几年的工作开创了使用基于MS的定量蛋白质组学，通过蛋白质丰度，细胞再定位和PTM来研究昼夜蛋白质功能。该技术已成为在昼夜节律领域的工具，以研究全球蛋白质组/PTM如何在时间上进行调节，从而全面了解生物钟如何调节生理和行为。这是进一步研究与昼夜节律相关的代谢、行为和认知障碍的先决条件，昼夜节律是一种经常与现代生活方式相关的疾病。因此，收购这种最先进的仪器将极大地促进时间生物学研究，并有利于和加强LMU的蛋白质组学社区。

Mass-spectrometry (MS) based quantitative proteomics is a powerful technology used to study tissue, cellular and sub-cellular proteomes as well as protein post-translational modifications (PTMs). This technology has revolutionized the quantification of protein levels and modifications for in many biological fields, amongst them chronobiology. The circadian clock is an endogenous timing system that regulates in a daily manner physiological functions and behavior. The circadian clock exerts its control by rhythmically modulating gene expression and protein function. While circadian transcription regulation is widely studied the understanding of circadian dynamics in protein function is in its infancy. My work in the last years has pioneered the use of MS based quantitative proteomics to study circadian protein function by means of protein abundance, cellular relocalization and PTMs. This technology has become instrumental in the circadian field to investigate how global proteomes/PTMs are temporally regulated to thus comprehensively understand how circadian clocks regulate physiology and behavior. This is a prerequisite to further investigate metabolic, behavioral and cognitive disorders associated with circadian asynchrony, a condition frequently associated with modern life styles. Therefore, the acquisition of this state of the art instrumentation will tremendously boost chronobiology research as well as benefit and strengthen the proteomics community at the LMU.