Signal Transduction and Control of T-cell function by Prolactin

催乳素已被证明在促进大鼠Nb 2前T淋巴瘤细胞中的G1/S转换期间诱导编码细胞周期蛋白D2和D3的基因的表达。分离编码这些大鼠细胞周期蛋白D2和D3的cDNA克隆并测序。细胞周期蛋白E、cdk 2、cdk 5和E2 F-1的基因也在同一阶段协同表达，表明存在共同的细胞机制来刺激这些基因。还发现，在相同的G1/S转换期，催乳素刺激TCR-nu基因表达4小时，并抑制TCR-α和β基因在Nb 2细胞。这些结果表明，催乳素参与调节哺乳动物T细胞的分化和增殖。

Prolactin has been demonstrated to induce the expression of the genes encoding cyclin D2 and D3 during promotion of the G1/S transition in rat Nb2 pre-T lymphoma cells. cDNA clones encoding these rat cyclins D2 and D3 were isolated and sequenced. The genes for cyclin E,cdk2, cdk5 and E2F-1 are also expressed synergistically at the same stage, suggesting the presence of a common cellular mechanism to stimulate these genes. It has also been found that, during the same G1/S transition phase, prolactin stimulates TCR-nu gene expression in 4 h and represses TCR-alpha and beta genes in the Nb2 cells. These results suggest that prolactin participates in the regulation of mammalian T-cell differentiation and proliferation.