Preparation of affinity latices and their application t of bioseparaton and cell activation.

两种生物特异性偶联均固定在亲水性强且具有固定结合位点的乳胶颗粒上。由此制备的亲和网可用于分离/纯化互补成分，或通过特异性结合反应激活细胞。在本研究中使用了以下夫妇：（DNA） n E4TF1b。TFIID TFIIAc。E4TF1小核核糖蛋白亚基。互补DNA。senseDNA antisenseRNAf。RGDS GPIIb/ gpiii . f， RGDS-亲和乳胶用于两个目的；从血小板膜提取纯化GPIIb/IIIa，并通过RGD-GPIIb/IIIa相互作用将白细胞活化转移到肌动蛋白丝。要获得高效的亲和链，必须发展以下三个环节；1.1 .合适载体颗粒的制备；2 .以合适的方式固定生物化合物；寻找在颗粒表面进行有效生物特异性反应的合适条件。详细研究了颗粒物理性质对亲和胶乳制备效率的影响。在所检测的颗粒中，聚苯乙烯核/聚甲基丙烯酸缩水甘油酯壳颗粒表现尤为优异。在一些系统中，生物功能分子的亚基或片段被固定在颗粒上。所得到的杂化颗粒具有足够的生物分离能力。在生物化合物和颗粒表面之间引入间隔链，提高了活性。

Either of biospecific couple was immobilizud to latex particles which were hydrophilic and have binding sites for the immobilization. Affinity latices thus prepared were employed for separation/purification of complementaey component, or cell activation via specific binding reaction. The following couples were used in this study ;a. (DNA) n E4TF1b.TFIID TFIIAc.Subunit of E4TF1 small nuclear Ribonucleo-Proteind.DNA Complementary DNAe.senseDNA antisenseRNAf.RGDS GPIIb/GPIIIaIn terms of f, RGDS-carrying affinity latex was used for two purposes ; GPIIb/IIIa purification from platelet membrane extract and leukocyte activation through RGD-GPIIb/IIIa interaction transferred to actin filament.The following three processed must be developed to get efficient affinity latices ; 1.Preparation of suitable carrier particle, 2.Immobilization of biocompounds in a proper mode, and 3.Search of suitable conditions to exhibit efficient biospecific reaction on particle surface.The effect of physical properties of particles on the efficiency of affinity latex was studied in detail.Polystirene-core/poly (qlycidyl methacrylate) shell particles were especially excellent among particles examined.In some systems, subunits or fragments of biofunctional molecules were immobilized on the particles.The resulting hybrid particles exhibited sufficient ability in bioseparation.Introduction of spacer chains between biocompound and particle surface improved the activity.