Studies on fine structure and function of rice photosynthetic apparatus

利用电镜和SDS-PAGE技术对水稻质体的相互转化进行了研究。从电镜观察，叶绿体到原质体的变绿过程是原质体到叶绿体的变绿过程的反向变化。在质体的结构变化过程中，不同的蛋白质被合成和分解。这表明这些蛋白与质体的结构支持和相互转化有关。为了分析油菜素内酯在光合作用中的作用，对植物细胞内14- c -表油菜素内酯的细胞间分布进行了研究。叶绿体部分放射性较高。其次，确定了14-C在叶绿体中的定位。类囊体部分的放射性在质体部分中最高，随时间的推移而增加。水稻培养细胞质体发育的一个显著特征是淀粉粒的产生与类囊体的形成同步。淀粉粒在质体发育过程中保留，但在去分化阶段消失。另一种是叶绿体去分化产生的无质体红蛋白前质体。

Interconversion of rice plastids was studies by electron microscopy and SDS-PAGE of plastid proteins. From electron microscopic observation, degreening process from chloroplasts to proplastids was a reverse change of greening from proplastids to chloroplasts. During the structural changes of plastids, distinctive proteins are synthesized and decomposed. It is suggested that these proteins are concernde in structural support and interconversion of plastids.To analysis a participation of brassinolide in photosynthesis, the intercellular distribution of 14-C-epibrassinolide in plant cells was investigated. The radioactivity was high in chloroplast fraction. Secondly, localization of 14-C in chloroplasts was determined. The radioactivity of thylakoid fraction was highest in plastid fraction and increased with the passage of time.A distinctive feature of plastid development in rice cultured cells i starch grain production synchronized with thylakoid formation. Starch grains remain through developmental process of plastids, but disappear in dedifferentiation stage. Another one is plastoglobule-less proplastids which resulted from chloroplast dedifferentiation.