Analysis of neural plasticity and behavioral diversity by the inducible method of single neuron gene expression

对声/振动诱发的斑马鱼幼虫快速逃逸行为分析表明，Mauthner （M-）细胞在快速逃逸的启动过程中起着至关重要的作用。在没有m细胞的情况下，非m回路可以触发快速向左或向右逃逸，而幼虫有时会表现出异常逃逸。利用热诱导绿色荧光蛋白（GFP）表达的转基因株系、边缘加工光纤和红外激光，成功地在斑马鱼胚胎和幼体的单个或多个目标神经元中诱导了绿色荧光蛋白的表达。

Behavioral analysis on sound/vibration-evoked fast escapes in larval zebrafish showed that Mauthner (M-) cell played a crucial role in the initiation of the escapes. In the absence of the M-cell, non-M circuits could trigger the fast escapes to the left or right, while larvae sometimes exhibited aberrant escapes. By using transgenic lines that drive heat-inducible expression of green fluorescent protein (GFP), edge-processed optical fibers and infrared laser, we succeeded in inducing GFP in targeted single or multiple neurons in embryonic and larval zebrafish brain.