Destruction of the multidrug efflux transporter on the plasma membrane by photodynamic treatment

作为癌症的治愈性治疗而建立的光动力疗法（PDT）通过在质膜或细胞质细胞器上产生活性氧而获得抗癌功效。在本研究中，我采用PDT诱导无明显细胞损伤，并研究PDT是否能降低顺铂或紫杉醇耐药HeLa细胞的耐药性程度，结果显示紫杉醇对紫杉醇耐药HeLa细胞的细胞毒性没有变化，而顺铂对顺铂耐药HeLa细胞的细胞毒性似乎增加。这些发现表明PDT是降低具有细胞质解毒机制如谷胱甘肽（GSH）或金属硫蛋白（MT）解毒途径的抗癌药物抗性细胞的抗性的有效项目。

Photodynamic therapy (PDT) established as a curative treatment for the cancer has acquired anticancer efficacy by producing reactive oxygen species on the plasma membrane or the cytoplasmic organelle. In this study, I employed PDT inducing no remarkable cell injury and investigate whether the PDT can reduce the degree of the resistance of cisplatin- or paclitaxel-resistant HeLa cells.As the results, the cytotoxicity of paclitaxel for the paclitaxel-resistant HeLa cells was unchanged, whereas, the cytotoxicity of cisplatin for the cisplatin-resistant HeLa cells seems to increase. These findings suggest that the PDT is effective item for the reducing resistance of the anticancer drug- resistant cells possessing the cytoplasmic detoxification mechanism such as Glutathione(GSH) or metallothionein(MT) detoxification pathway.