Development of visualization technique of cancer cell by click chemistry

本课题主要研究癌细胞可视化技术的发展。利用光在空间和时间上的高分辨率，以有机化学为基础合成的荧光探针被用于观察癌细胞。利用有机小探针分子对癌细胞进行特异、高效的点击反应，点亮癌细胞，实现对癌变肿瘤的实时检测。我们合成了以炔为示踪剂的核酸和糖衍生物。我们进一步开发了一种具有叠氮基团的香豆素分子作为荧光基团。原荧光基团香豆素没有荧光，而点击反应会产生强烈的荧光。然后，我们利用荧光团片段作为点亮报告子，基于点击反应绘制染色体DNA，并将清晰的染色体可视化成多色。该方法已成功应用于细胞中信号分子衍生物8-叠氮基cAMP的检测。

The research project focuses on the development of visualization technique of cancer cell. Utilizing the high resolution of light on spatial and temporal, the fluorescent probe that synthesized on the basis of organic chemistry is used to visualize cancer cells. To light up the cancer cells by small organic probe molecules with specific and efficient click reaction on cancer cells, cancerous tumor can be detected in real time. We synthesized the nucleic acid and sugar derivatives having alkyne as a tracer. We further developed a coumarin molecule having an azide group as a profluorophore. The profluorophore coumarin has no fluorescence, whereas click reaction will result in a strong fluorescence. We then used the profluorophore moiety which serve as a light-up reporter to painting chromosome DNA based on click reaction and visualized the clear chromosomes in multicolor. The method has been successfully applied to detect the signaling molecule derivative 8-azido cAMP in cells.