Development of site-specific conversion method of nucleobase in mRNA

本研究旨在建立信使RNA中胞嘧啶核碱基转化为尿嘧啶核碱基的方法。为了实现该方法，设计了一类新的修饰寡核苷酸-磺酸偶联寡核苷酸，并进行了合成。虽然在本研究期间研究了在各种条件下使用合成的寡核苷酸在2 ′-O-甲基-RNA内的胞嘧啶核碱基的转化反应，但我们未能成功开发转化方法。本研究为该方法的进一步发展提供了有益的参考。

In this study, we aimed at the development of the method for conversion ofcytosine nucleobase into uracil one in messenger RNA. In order to implement the method, sulnic acid-conjugated oligonucleotides were designed as a new class of modified oligonucleotides, and their syntheses were achieved. Although the conversion reaction of cytosine nucleobase within 2'-O-methyl-RNA using the synthesized oligonucleotides under various conditions was investigated during this research period, we could not succeed in the development of the conversion method. However, this reseach provided useful information for the development of the method in future.