Development and Application of Algae Photosynthesis Inhibition Assay

为了对污水和环境水体进行适当的管理，必须对其毒性进行评价。由于样品中可能含有多种化学物质，然而，通过确定单个化学物质的浓度和毒性，很难得出这些样品的总体毒性。本研究的具体目的是开发和实际应用藻类光合作用抑制法检测水样的整体毒性，该方法只需要少量的测试溶液，是快速可靠的生物检测方法之一。本研究最终选择了两种有用的光合藻类，对其进行了适当而详细的方法研究和开发，并通过城市污水处理厂的流出物和日本琵浪湖和美国Suwanee河的天然有机物质（NOM）来证实其适用性。

For the proper management of effluents and environmental water bodies, the evaluation of their toxicity is essential. As a variety of chemicals may be contained, it is however difficult to come up with the overall toxicity of those samples with identifying the concentration and toxicity of individual chemicals. The specific objectives of this research were set to the development and actual application of algal photosynthesis inhibition assay for the detection of whole toxicity of water samples, which requires only small volume of test solution with being one of the fast and reliable bioassays. In this research, two useful photosynthetic algae were eventually selected, its appropriate and detail methodology was investigated and developed, and its applicability was confirmed with the effluent of a municipal wastewater treatment plant and the NOM (Natural Organic Matter) of Lake Biwa in Japan and Suwanee River in USA.