Identification of nascent polypeptides with novel functions

我们试图识别在合成过程中起作用的新型新生多肽链。在我们的筛选过程中，我们意识到绝大多数细胞蛋白在翻译延伸期间经历了短暂的停顿。这些结果挑战了我们对翻译延伸的传统观点，同时，他们展示了太多新的调控新生链的候选物。对先前确定的调控新生链进行基因改造，可以发明新的传感器。原生传感器也被认为是研究蛋白质膜插入机制的有力工具。

We tried to identify novel nascent polypeptide chains that function during being synthesized. In the course of our screening, we are realizing that vast majority of cellular proteins undergoes transient pauses during translation elongation. These results are challenging our conventional view of translation elongation, while, at the same time, they are displaying too many candidates for novel regulatory nascent chains. Genetic modification of previously-identified regulatory nascent chain allowed to invent novel sensors. Original native sensors also found to function a powerful research tool to investigate molecular mechanisms of a protein membrane insertion machinery.