Development of High sensitive and high-resolution detection of extra-celluar ATP in mouse and searching ATP signal in mouse embryo

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To detect the low density and extra-celluar ATP, ATeamL was developed. ATeamL was constructed in FRET-based ATP indicator by genetically linking GFP and Kusabira Orange with epsilon subunit , which was derived from Bacillus (high affinity to ATP). ATeamL, connected to membrane anchoring sequence, was displayed on extra-celluar region in HeLa cell. Now, I generate the transgenic mouse using this construct to be able to detect the extra-celluar ATP in vivo.