Development of the recognition molecule for 8-oxo-rG in RNA

在这项研究中，我们开发了新的核苷类似物来识别核酸的损伤，以建立基于应激的新的疾病诊断。8-oxo-G是鸟嘌呤的氧化损伤碱基，其在单链RNA中的性质尚不清楚。因此，本工作的目的是发展一种序列特异性的检测RNA中8-oxo-Rg的分子和方法。我们开发了一种新的RNA型8-oxoG的合成方法。并发现了新的核苷类似物2-氨基-ADAP和2‘-OME-ADAP。这些类似物能够识别和检测RNA中的8-oxo-Rg。

In this study, we developed the novel nucleoside analogues for the recognition of the damage to the nucleic acid in order to establish a new diagnosis of disease based on the stress. 8-Oxo-G is the oxidative damage base of guanine, and it is unknown the properties of it in single-stranded RNA. Thus, the purpose of the present work was developed a sequence specific detection molecules and methods of 8-oxo-rG in RNA. We developed a new synthesis method of RNA-type 8-oxoG. And we found the novel nucleoside analogues, 2-amino-Adap and 2'-OMe-Adap. These analogues could recognize and detect the 8-oxo-rG in RNA.