Biological application of micro-dissection techniques for scanning electron microscopy

在本研究中，我们介绍了一种可用于立体扫描电子显微镜(3D-SEM)的显微解剖技术，并将该技术应用于细胞和组织的三维超微结构的研究，开发了适用于3D-SEM中两个微操作器解剖样品的制备方法。我们还使用了改进的带针的机械手、微型剪刀和微型钳子。使用这些微操作器，我们成功地在3D-SEM中解剖了一些组织样本(如肾脏、内耳等)。因此，3D-SEM中的扫描电子显微镜解剖技术对于细胞和组织的形态研究是非常有用的。

In the present study, we introduced a microdissection technique which can be used in a stereoscopic scanning electron microscope (3D-SEM) and applied this technique to the study on the three-dimensional ultrastructure of cells and tissues.We developed preparation methods suitable for dissecting samples by the two micro-manipulators in the 3D-SEM. We also used the improved manipulators with a needle, micro-scissors and micro-forceps. Using these micro-manipulators, we succeeded in dissecting some tissue samples(e.g, kidney, inner ear, etc) in a 3D-SEM. Thus, the SEM-dissection technique in a 3D-SEM is very useful for the morphological study on cells and tissues.