Identification of multi-level regulation of gene expression that determines B cell development

我们发现，在B细胞分化为浆细胞的过程中，组蛋白变体的沉积明显减少。我们还通过CHIP-SEQ和mRNA-SEQ证实了组蛋白变异体与分化过程中表达的基因的调控元件结合。此外，His ton变异体在B细胞中的结构性表达导致浆细胞生成受到抑制。这些结果表明，B细胞的分化受染色质结构的动态变化控制。

We found that the deposition of histone variant was markedly decreased during B cell differentiation into plasma cells. We also confirmed by chip-seq and mRNA-seq that the histone variant bind to the regulatory element of genes that will be expressed during differentiation. Furthermore, constitutive expression of his ton variant in B cells resulted in the inhibition of plasma cell generation. These findings suggest that B cel differentiation is controlled by dynamic change of chromatin structure.