Tumor specific delivery of nucleic acid using patient's dendritic cell-derived exosome

为了改善儿童癌症的预后，需要创新的治疗方法。对几种肿瘤抑制基因microRNAs进行了鉴定，并对其临床应用提出了建议。外切体在microRNA的细胞间通讯中起着重要作用。我们研究了外切体是否可以用来向儿科癌细胞运送肿瘤抑制基因microRNA。结果表明，儿科肿瘤细胞分泌外切体，外切体来源的microRNA的表达模式与细胞内microRNA的表达模式相似，外切体具有细胞间通讯的功能。我们的研究结果表明，外切体可以作为传递microRNA的手段。

Innovative therapeutics is required to improve prognosis of pediatric cancer. Several tumor suppressor microRNAs are identified and clinical application of microRNAs are suggested. Exosome plays important roles in intercellular communication of microRNA. We examined if exosome can be used to deliver tumor suppressor microRNA to pediatric cancer cells. It is revealed that pediatric cancer cells secrete exosome, that expression pattern of exosome-derived microRNA is similar to that of cellular microRNA and that exosome functions as menas of intercellular communication. Our findings suggested that exosome can be used as means of microRNA delivery.