Application of pseudopodia proteomics using excimer laser ablation for investigation of lung cancer invasion

伪足蛋白的鉴定有助于进一步了解肿瘤细胞的恶性表型和新的治疗策略。将全细胞和伪足部分中的蛋白质分别溶解，用高灵敏的荧光染料标记，然后用双向差示凝胶电泳法分离。在观察到的2508个蛋白质点中，211个在整个细胞和伪足部分之间有不同的强度(相差四倍以上，P<0.05)。免疫组织化学检测证实了RAB1A、HSP90B、TDRD7和波形蛋白等新的伪足定位蛋白的定位。以往的研究表明，这四种蛋白可能在细胞迁移过程中发挥作用。这种方法将提供对伪足分子细节的洞察，并进一步了解肿瘤细胞的恶性表型和肺癌的新治疗策略。

Identification of pseudopodia proteins leads to a further understanding of malignant phenotypes of tumor cells and novel therapeutic strategies. Proteins in whole cells and pseudopodia fractions were individually solubilized, labeled with a highly sensitive fluorescent dye, and separated using two-dimensional difference gel electrophoresis. Among 2508 protein spots observed, 211 had different intensity between whole cells and pseudopodia fractions (more than fourfold differences and P-value of <0.05). The localization of novel pseudopodia-localizing proteins such as RAB1A, HSP90B, TDRD7, and vimentin was confirmed using immunohistochemical examinations. The previous studies demonstrated that these four proteins may function in the cell migration process. This method will provide insights into the molecular details of pseudopodia and a further understanding of malignant phenotypes of tumor cells and novel therapeutic strategies for lung cancer.